Phospho-h2ax ser139

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August undefined, 2024

WebPhospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb detects endogenous levels of H2A.X only when phosphorylated at serine 139. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X. WebAnti-phospho-H2A.X (Ser139) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-H2A.X (Ser139) also known as histone 2 H2ac, histone IIa & has been validated in IP & WB. Immunoprecipitation: A previous lot of this antibody successfully immunoprecipitated phosphorylated H2A.X as determined by an independent laboratory. …

Phospho-Histone H2A.X (Ser139) Antibody - Cell …

WebJan 13, 2011 · Herein, we report that EGF strongly induces histone H2AX phosphorylation at Ser139 and at a newly identified phosphorylation site, Ser16, in vitro and ex vivo, and both phosphorylations are mediated by RSK2.Phosphorylation of histone H2AX by RSK2 prevented histone H2AX degradation, and histone H2AX could block cell transformation … WebFeb 2, 2024 · (D, E) IHC staining of phospho‐BRCA1 (Ser1423) and phospho‐H2AX (Ser139) on MM samples (N ≧ 7). Compared with the wild‐type, the Brca1 Mut group revealed similar fractions of EM and no malignancy, but a higher fraction of SM, as well as a lower proportion of BM (Figure 1D ), indicating the promotion of epithelial–mesenchymal ... toazted josh groban

Anti-phospho-Histone H2A.X (Ser139) Antibody, clone 6L16, …

WebFeb 17, 2024 · a. Preparation of rabbit anti-phospho-H2AX (Ser139) antibody: Briefly spin the vial of rabbit anti-phospho-H2AX (Ser139) (Item C-1). Add 100 µl of 1X Assay Diluent … WebAnti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301 is a highly specific mouse monoclonal antibody, that targets Histone H2A.X & has been tested in western blotting, ICC, ChIP & Immunof More>> MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. SDS CoA WebPhosphorylated H2A.X promotes DNA repair and maintains genomic stability. The 2F3 monoclonal antibody reacts with phosphorylated human H2A.X (Ser139) and has been shown to be useful for Western blotting, immunofluorescence and flow cytometry. Product Details Technical data sheet Product Details Verified Reactivity Human, Mouse Antibody … tob003

Phospho-Histone H2A.X (Ser139) AlphaLISA SureFire Ultra …

WebNov 15, 2024 · RayBio® Phospho-H2AX (S139) and Total H2AX ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated H2AX protein in your experimental model system, you can verify pathway activation in your cell lysates. WebIntracellular staining protocol for Anti-H2A.X-Phosphorylated (Ser139) Antibody for Flow Cytometry. 1. Prepare 70% absolute ethanol. Chill solution by storing at -20°C. 2. Prepare cells of interest. 3. Wash 1X with PBS, centrifuge at 350g for 5 min. 4. penn state early childhoodWebIntracellular staining protocol for Anti-H2A.X-Phosphorylated (Ser139) Antibody for Flow Cytometry. 1. Prepare 70% absolute ethanol. Chill solution by storing at -20°C. 2. Prepare cells of interest. 3. Wash 1X with PBS, centrifuge at 350g for 5 min. 4. penn state early admission

"WebApr 14, 2024 · Abstract. Background: HER3, a member of the ERBB family of receptor tyrosine kinases that activates multiple oncogenic signaling pathways, is overexpressed in 50-70% of breast cancers (BC). HER3 mRNA expression is highest in luminal (ER+) breast tumors. Approximately 30% of ER+ breast tumors are de novo resistant to tamoxifen. … " - Phospho-h2ax ser139

Phospho-h2ax ser139

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WebAnti-gamma H2A.X (phospho S139) antibody [EP854 (2)Y] See all gamma H2A.X primary antibodies Description Rabbit monoclonal [EP854 (2)Y] to gamma H2A.X (phospho S139) Host species Rabbit Tested applications Suitable for: IP, IHC-P, WB, ICC/IF, Dot blot more details Species reactivity Reacts with: Mouse, Rat, Human Predicted to work with: Sheep WebThe AlphaLISA SureFire Ultra Phospho-Histone H2A.X (Ser139) assay kit is an immunoassay for quantitative detection of phosphorylated Histone H2A.X in cellular lysates. We are now two standalone organizations Life Sciences & Diagnostics and Analytical & Enterprise Solutions

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WebNov 15, 2024 · RayBio® Phospho-H2AX (S139) and Total H2AX ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important … WebThis Histone H2AX phospho Ser139 antibody was raised against a peptide including phospho-serine 139 of histone H2AX. Buffer 10 mM sodium phosphate pH 7.5, 150 mM NaCl, 30% glycerol, 0.035% sodium azide. View our Guide to Histone Modifications and Biological Function. Histone H2AX phospho Ser139 antibody tested by …

WebPhospho-gamma-H2AX (Ser139) Antibody (IHC-00059) in IHC Detection of human gamma H2AX by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-gamma H2AX (Cat. No. IHC-00059 Lot6) used at a dilution of 1:250. Detection: DAB. Product Details Product Specific Information WebBecause phosphorylation of H2AX at Ser 139 (γ-H2AX) is abundant, fast, and correlates well with each DSB, it is the most sensitive marker that can be used to examine the DNA …

WebBethyl Laboratories Anti-Phospho-gamma-H2AX (Ser139) Polyclonal, Catalog # A300-081A. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF) and Immunohistochemistry … WebThis Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-gamma H2A.X. An anti-pan gamma H2A.X antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and gamma H2A.X present in a sample is bound to the wells by the immobilized antibody.

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WebDescription Histone H2AX is a variant of the nucleosome core histone H2A and is phosphorylated at Ser139 in response to DNA damage. Histone H2AX phosphorylation is considered a specific reporter of double-strand DNA breaks. The protein is also referred to as γH2AX when phosphorylated at Ser139. tob.003WebThis Histone H2AX phospho Ser139 antibody was raised against a peptide including phospho-serine 139 of histone H2AX. Buffer 10 mM sodium phosphate pH 7.5, 150 mM … penn state early applicationWeb公司专业供应的培养基，dragon牙膏稀释缓冲液使用说明书现货直销，质量有保障，款到当天可发货，免费快递送货上门，欢迎 ... penn state early childhood training onlineWebProduct Specific Information. Description: The CR55T33 monoclonal antibody recognizes phosphorylated serine 139 of human and mouse H2AX. H2AX is a member of the H2A … tob 0224WebPhospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb detects endogenous levels of H2A.X only when phosphorylated at Ser139. Species Reactivity: Human, Mouse, Rat, Monkey … penn state early childhood education trainingWebMar 31, 2016 · Get better batch-to-batch reproducibility with a recombinant antibody. Anti-gamma H2A.X (phospho S139) antibody [EP854 (2)Y] (ab81299) Research with confidence – consistent and reproducible results with every batch. Long-term and scalable supply – powered by recombinant technology for fast production. tob 014xWebPhosphorylated H2A.X promotes DNA repair and maintains genomic stability. The 2F3 monoclonal antibody reacts with phosphorylated human H2A.X (Ser139) and has been shown to be useful for Western blotting, immunofluorescence and flow cytometry. Product Details Technical data sheet Product Details Isotype Control FITC Mouse IgG1, κ Isotype … tob 0131