Pherd20t
Web... pHERD20T is an arabinose-inducible vector based on pUCP20T that supports replication in P. aeruginosa and shares characteristics of the pUCP vector family. Web1. okt 2024 · PAO1 harbouring pHERD20T‐dCas9 or pHERD20T‐dCas9‐prtR were used in the tests. (d) Plating efficiencies of 5 × 10 8 /ml bacterial cells (PAO1 harbouring …
Pherd20t
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Web22. dec 2024 · Patients and methods: The shuttle vector pHERD20T containing an arabinose inducible promoter was used to construct the CRISPRi system. Alterations in cellular … Webpet15b载体简介. pET-15b载体具有N端His标签和thrombin酶切位点，用于周质腔蛋白表达。. 独特的酶切位点在载体图谱中被标注出来。. 需要注意的是，这些载体序列是依据载 …
WebEscherichia-Pseudomonas shuttle vector pHERD20T. Disclaimer: The NCBI taxonomy database is not an authoritative source for nomenclature or classification - please consult …
Web16. dec 2012 · The pHERD20T vector was digested with EcoRI and HindIII and then infused using the IN-Fusion EcoDry cloning kit (Clontech) following the manufacturer’s instructions. A previously described31 environmental strain of P. aeruginosa was used as a template to amplify the four cas genes of the Type I-C CRISPR–Cas system genes (cas3, cas5, cas7 and cas8). The genes were cloned into … Zobraziť viac Transformations of P. aeruginosa, E. coli, P. syringae and K. pneumoniae strains were conducted using standard electroporation … Zobraziť viac PAO1IC was grown overnight at 37 °C in LB media. Then 150 μl of overnight culture was added to 4 ml of 0.7% LB top agar and spread on 1.5% LB agar plates supplemented … Zobraziť viac The isogenic DMS3macrIIA4 and acrIC1 phages were constructed using previously described methods60. A recombination cassette, pJZ01, was constructed with homology to the … Zobraziť viac
Web(A) AlgUA61V retained the function of inducing alginate production. The wild type algU gene of PAO1 and its variant of CF149 were cloned into pHERD20T, and conjugated into …
WebPseudomonas aeruginosa is a widely distributed non-fermentative Gram-negative opportunistic pathogen that is often responsible for nosocomial infections. Gene … refinery agency manchesterWeb5. sep 2024 · A recombination cassette consisting of genes 34 to 38 of phage JBD30 (anti-CRISPR locus with large flanking regions) in plasmid pHERD20T was previously generated … refinery air watchWeb3. mar 2016 · Cyclic diguanosine monophosphate (c-di-GMP) is one of the most important bacterial second messengers that controls many bacterial cellular functions including … refinery albertonWebNational Center for Biotechnology Information refinery alabamaWeb22. aug 2013 · Strains containing pHERD20T-algU WT (PAO1), pHERD20T-algU A61V (CF149), and pHERD20T were streaked on PIA plates supplemented with 300 µg/ml of … refinery air coolerWeb20. okt 2024 · Mutations were confirmed by sequencing. pHERD20T-fleN WT, pHERD20T-vector, pHERD20T-fleN L263W, and pHERD20T-fleN TP255 were electroporated in the … refinery amine unitWeb25. jan 2013 · pHERD20T: Arabinose-inducible/glucose-repressible P BAD-based shuttle vector; Ap r: 19: pHERD20T-wbpM O1-Flag: pHERD20T containing the coding region of … refinery alky unit