Hifiscript cdna
Webwas used for reverse transcript using the HiFiScript cDNA Synthesis kit, according to the manufacturer's protocol. ChIPBase (rna.sysu.edu.cn/chipbase) was used to screen for lncRNAs associated with TBI, including 75 upregulated and 22 downregulated lncRNAs. Among these, transcription factors involved in the inflammatory response were screened, WebThe High Capacity cDNA Reverse Transcription Kit (formerly the High Capacity cDNA Archive Kit) delivers extremely high quality first-strand cDNA from total RNA. The kit was …
Hifiscript cdna
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Web2）rnaseh活性：在反应过程中切割rna：cdna杂合链中的rna模板。降低此活性，可避免在合成较长cdna之前模板rna被降解掉。 3）dna指导的dna聚合酶活性：以反转录合成的第 … Web本产品是专为两步法RT-PCR第一步实验配制的cDNA第一链合成试剂盒。该试剂 盒中使用的HiFiScript逆转录酶是M-MLV由来的新型高效逆转录酶，新颖突变位点大幅 提升酶的转 …
WebHiFiScript cDNA Synthesis Kit. This product is a first-strand cDNA synthesis kit designed for the first step of two-step RT-PCR. The HiFiScript reverse transcriptase used in the … Web技术领域. 本发明涉及耐药相关基因gabrp在结直肠癌耐药中的应用，属于生物医药领域。 背景技术. 结直肠癌(crc)是全球第三大常见癌症，也是第二致命的癌症，2024年约有190万例新发病例和超过935，000例死亡。
Web6 de ago. de 2024 · The biological function of the aromatase gene (cyp19a) is to transform testosterone into estradiol, which is involved in ovarian differentiation and development. In the present study, in order to explore the role of cyp19a in sex differentiation of Culter alburnus, the full-length cDNA sequence was obtained, and the relationship between … Web本产品是专为两步法RT-PCR第一步实验配制的cDNA第一链合成试剂盒。该试剂盒中使用的HiFiScript逆转录酶是M-MLV由来的新型高效逆转录酶，新颖突变位点大幅提升酶的转 …
Web19 de jul. de 2024 · Subsequently, the total RNA was reverse transcribed into cDNA with the use of HiFiscript cDNA Synthesis Kit according to the manufacturer’s instructions. Quantitative real-time PCR was performed using 2× Fast Super EvaGreen® qPCR Mastermix on a LightCycler96 Real-Time PCR system (Roche, Basel, Switzerland).
WebFeatures. Rapid genomic DNA deletion: With the gDNA remover, it takes only 2 minutes to remove genomic DNA. Rapid reverse transcription: It takes only 15 minutes to obtain the … sport additum bayern abiturWebThe kit removes genomic DNA in 2 minutes at 42°C. Meanwhile, because the reverse transcription reagents contain components that inhibit gDNA Remover, the sample … shells learning centerWebComplementary DNA (cDNA) was synthesized with the reverse transcription enzyme SuperScript II (Invitrogen; Thermo Fisher Scientific, Inc.) together with reverse transcription primers at 50˚C for 15min and 85˚C for 5sec. cDNA was then amplified using a HiFiScript cDNA Synthesis Kit (CoWin Biosciences) using an ABI Q5 RT‑PCR System (Applied ... shell sleep 0.5Webcentration, HiFiScript cDNA Synthesis Kit (CW2569M, CWBIO, China) and miRNA cDNA Synthesis Kit (CW2141S,CWBIO,China)wereusedreversetranscription with a 20μL reverse transcription reaction system. SYBR-Green PCR Master Mix (CW2601S, CWBIO, China) was used for PCR amplification using the 30μL amplification shell sleep 1分钟WebDescripción. The iScript cDNA synthesis kit is a sensitive and easy-to-use first-strand cDNA synthesis kit for gene expression analysis using real-time qPCR. This two-tube kit is … shell sleep 10秒WebWhen the cells grew to 80% confluence, they were cultured for 48 h in media containing different concentrations of glucose: 0 (Control), 5.5, 15 and 25 mmol/l. Total RNA from multiple cells was extracted using TRIzol reagent and reverse transcribed into cDNA using the HiFiScript cDNA synthesis kit, according to the manufacturer's protocol. shell sleep 100msWebThis particular kit also includes 5 tubes of RNase inhibitor (200 µL each). The High Capacity cDNA Reverse Transcription Kit delivers extremely high-quality, single-stranded cDNA from 0.02 to 2 µg total RNA. Reactions … shell sleep 10